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KMID : 0364820120480040309
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Korean Journal of Microbiology
2012 Volume.48 No. 4 p.309 ~ p.313
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Rapid Detection of Lactobacillus Genus Inhabiting in Vagina of 20’s Healthy Women Using Multiplex PCR
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Oh Cho-Rong
Cho Hong-Bum
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Abstract
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The purpose of this study is to simultaneously detect and identify specific genus of Lactobacillus distributed in
vagina of healthy women in their twenties by using multiplex primer. Vaginal fluid samples were taken from 166
women who were healthy and did not have vaginosis symptoms caused by bacterial infection. Six species of lactic
acid bacteria belong to Lactobacillus genus that are known to inhabit in healthy vagina were selected to make a
species-specific multiplex primer. Multiplex primer I was specified to selectively detect L. iners, L. crispatus, L.
gasseri and multiplex primer II was specified to selectively detect L. acidophilus, L. jensenii, L. fermentum. L.
crispatus (77%) was most frequently detected, and L. acidophilus (57%) and L. jensenii (57%) were relatively higher
than others. Although the proportion of L. iners (59%) was a little higher than those of L. acidophilus and L. jensenii,
this should be further validated in healthy women?s vagina to be sure since they often appear in women having
bacterial vaginosis and/or during the antibiotic therapy. Conclusively, the multiplex PCR technique using the
species-specific primer could a useful tool to predict variation of vaginal health condition and process of recovery
from vaginal disease
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KEYWORD
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Lactobacillus sp., bacterial vaginosis, multiplex PCR
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